DNA evidence constitutes opinion
evidence as contemplated under Section 45 of the Indian Evidence Act, and like
any other opinion evidence, its probative value varies from case to case. The courts must evaluate DNA evidence with appropriate scrutiny
while considering its scientific foundation and procedural compliance.
DNA Profiling: Scientific Methodology
and Process
Nature of
DNA Evidence
·
DNA
(Deoxyribonucleic acid) is genetic material present in cell nuclei, composed of
four nucleotides: Adenine (A), Guanine (G), Thymine (T), and Cytosine (C).
·
Only 0.1%
of DNA differs between individuals, with forensic scientists analyzing variable
regions to generate unique DNA profiles
·
Current
genetic markers of choice for forensic purposes are Short Tandem Repeats (STRs)
DNA
Profiling Methodology
The DNA profiling process involves four sequential steps:
·
Isolation,
purification and quantitation of DNA
·
Amplification
of selected genetic markers
·
Visualizing
fragments and genotyping
·
Statistical
analysis and interpretation
Statistical Analysis and Interpretation
Standards
Three
Possible Outcomes of Profile Comparison:
·
Match: DNA profiles from two samples are indistinguishable
·
Exclusion: Profile differences indicate different biological sources
·
Inconclusive: Data insufficient for definitive
conclusion
Random
Match Probability (RMP):
Statistical calculations must support any declared "match" by
estimating the frequency of the particular DNA profile in a population.
Collection and Preservation
Requirements
Legal and
Scientific Prerequisites
DNA evidence must be properly documented, collected, packaged, and preserved to
meet legal and scientific requirements for court admissibility. Contamination risks require heightened attention during:
·
Location
and identification of biological specimens
·
Collection
and packaging procedures
·
Chain of
custody maintenance
·
Laboratory
transmission protocols
Evidentiary Standards and Judicial
Scrutiny
Quality
Control Requirements
Courts have emphasized that DNA profile validity and reliability depend on
quality control and quality assurance procedures in the laboratory. The mere exhibition of DNA reports without examining their
underlying basis renders such evidence "highly vulnerable".
Burden of
Proof Standards
·
DNA
evidence may be more useful for investigation purposes than for raising
presumptions of identity in court
·
Courts
must examine whether DNA techniques were reliably applied by experts
·
Possibility
of sample tampering during storage periods affects evidential weight
Precedential Guidelines
International
Standards:
Following R v. Dohoney & Adams, courts should require:
·
Scientist
testimony on DNA comparisons with random occurrence ratio calculations
·
Prosecution
disclosure of calculation methodologies to defense
·
Defense
expert access to databases underlying the calculations
Judicial
Observations: DNA
testing provides "powerful new evidence unlike anything known before"
and can determine biological tissue matches "with near certainty,"
while simultaneously serving to exonerate wrongly convicted individuals and
confirm valid convictions.
Legal Principle Established
DNA evidence, while scientifically
advanced and potentially conclusive, requires strict adherence to collection
protocols, chain of custody procedures, and quality control standards. Courts
must scrutinize both the scientific methodology employed and the procedural
compliance achieved before according probative value to such evidence in
criminal proceedings.
IN THE SUPREME COURT OF INDIA
Criminal Appeal Nos. 611, 612-613 and 614-615 of 2022